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qiaquick pcr purification kit  (Qiagen)


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    Structured Review

    Qiagen qiaquick pcr purification kit
    Setup of the protocol analysis summarized in various steps.
    Qiaquick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 61952 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qiaquick+purification+kit/QIAquick+PCR+Purification+Kit/pmc13667942-67-21-25
    Average 99 stars, based on 61952 article reviews
    qiaquick pcr purification kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Development and Validation of PCR-Based Ancient DNA Analysis for Italian and Western Balkan Archaeological Collections"

    Article Title: Development and Validation of PCR-Based Ancient DNA Analysis for Italian and Western Balkan Archaeological Collections

    Journal: Balkan Journal of Medical Genetics : BJMG

    doi: 10.2478/bjmg-2026-0008

    Setup of the protocol analysis summarized in various steps.
    Figure Legend Snippet: Setup of the protocol analysis summarized in various steps.

    Techniques Used: Analysis, Sequencing, DNA Extraction, Sample Prep, Irradiation, Lysis, DNA Purification, Purification, DNA Sequencing, Cloning, Genotyping

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Detection of Enterocytozoon hepatopenaei in pond-cultured Litopenaeus vannamei shrimp: Unveiling the epidemiological significance of some invasive macrofauna, synergistic diagnostic approach and treatment trial.
    Article Snippet: Hepatopancreatic microsporidiosis (HPM) is protozoal disease caused by Enterocytozoon hepatopenaei (EHP), causing remarkable economic losses in the shrimp farming due to associated mortalities as well as size variations.. The current study aimed to investigate the roles of some invasive macrofauna in the epidemiology of EHP, analyze the associated hepatopancreatic pathologies and check the efficacy of a novel field treatment for the EHP affected pond.. A total of 100 L. vannamei were randomly collected from the affected pond before and after treatment trial.

    Article Title: Anti-ADAMTS13 antibody and use thereof
    Article Snippet: .. TABLE 2 Primer 1 ggggcggccgcAGAGTGGC SEQ ID NO: 51 CATTACGGCCGGG Primer 2 GGGGAATTCtcatttaccc SEQ ID NO: 52 ggagaccgggagatgg The PCR sample was applied to QIAquick Purification Kit (Qiagen N.V.) for the removal of unreacted primers and enzyme to obtain a purified PCR product. .. The purified PCR product obtained by purification was treated with a restriction enzyme, was separated and purified by agarose gel electrophoresis, and then integrated in a vector. pQEFIP vector (retrovirus vector obtained by using pQCXIP (Takara Bio Inc.) as a backbone in which CMV promoter was replaced with human EF1 alpha promoter) was used for the preparation of a vector containing the heavy chain gene, and pQEFIN vector (retrovirus vector obtained by using pQCXIN (Takara Bio Inc.) as a backbone in which CMV promoter was replaced with human EF1 alpha promoter) was used for the preparation of a vector containing the light chain gene.

    Article Title: Community based countrywide analysis of lactase persistence related genetic variants and their correlation with digestive symptoms in Libya.
    Article Snippet: Genomic DNA was extracted from buccal swabs using Isohelix Buccal DNA Isolation Kit (Cell Projects) according to the manufacturer’s instructions and it was checked by agarose gel electrophoresis. .. Excess salts, primers, and dNTPs were removed by using QIAquick Purification Kit (Qiagen) according to the manufacturer’s protocol before PCR amplification. ..

    Article Title: DNA Barcoding and Allele-Specific PCR Discrimination of Glasswort Ecotypes from Apulia Region (Southern Italy)
    Article Snippet: .. MatK and rbcL fragments were purified from 100 μL of PCR products using the QIAquick purification Kit (Qiagen, Hilden, Germany) and bidirectionally sequenced at the Eurofins Genomics Sequencing Service (Ebersberg, Germany) with the same PCR primers. ..

    Amplification:

    Article Title: Detection of Enterocytozoon hepatopenaei in pond-cultured Litopenaeus vannamei shrimp: Unveiling the epidemiological significance of some invasive macrofauna, synergistic diagnostic approach and treatment trial.
    Article Snippet: Hepatopancreatic microsporidiosis (HPM) is protozoal disease caused by Enterocytozoon hepatopenaei (EHP), causing remarkable economic losses in the shrimp farming due to associated mortalities as well as size variations.. The current study aimed to investigate the roles of some invasive macrofauna in the epidemiology of EHP, analyze the associated hepatopancreatic pathologies and check the efficacy of a novel field treatment for the EHP affected pond.. A total of 100 L. vannamei were randomly collected from the affected pond before and after treatment trial.

    Article Title: Community based countrywide analysis of lactase persistence related genetic variants and their correlation with digestive symptoms in Libya.
    Article Snippet: Genomic DNA was extracted from buccal swabs using Isohelix Buccal DNA Isolation Kit (Cell Projects) according to the manufacturer’s instructions and it was checked by agarose gel electrophoresis. .. Excess salts, primers, and dNTPs were removed by using QIAquick Purification Kit (Qiagen) according to the manufacturer’s protocol before PCR amplification. ..

    Purification:

    Article Title: Detection of Enterocytozoon hepatopenaei in pond-cultured Litopenaeus vannamei shrimp: Unveiling the epidemiological significance of some invasive macrofauna, synergistic diagnostic approach and treatment trial.
    Article Snippet: Hepatopancreatic microsporidiosis (HPM) is protozoal disease caused by Enterocytozoon hepatopenaei (EHP), causing remarkable economic losses in the shrimp farming due to associated mortalities as well as size variations.. The current study aimed to investigate the roles of some invasive macrofauna in the epidemiology of EHP, analyze the associated hepatopancreatic pathologies and check the efficacy of a novel field treatment for the EHP affected pond.. A total of 100 L. vannamei were randomly collected from the affected pond before and after treatment trial.

    Article Title: Virulence determinants and multidrug resistance of emerging Salmonella enterica subspecies salamae in broiler chickens: a potential zoonotic concern
    Article Snippet: .. Amplicons were purified using a QIAquick purification kit (Qiagen, Germany). .. Afterwards, sequencing was performed on an ABI 3500 Genetic Analyzer (Applied Biosystems, USA) using the Big Dye Terminator V3.1 kit.

    Article Title: Evaluation of a Customized RDB-FTH Panel for β-Globin Variants in a Malaysian Tertiary Center.
    Article Snippet: β-thalassemia is one of the most prevalent autosomal recessive genetic disorders in Malaysia, with an estimated prevalence of 4.5%, encompassing asymptomatic carriers, intermedia and transfusion-dependent β-thalassemia.. Molecular diagnosis has become increasingly important to correlate phenotype to genotype, guiding management, and facilitating genetic counseling, particularly in the prenatal diagnostic setting.. this study evaluated a rapid reversed dot-blotbased flow-through-hybridization (RDB-Fth) assay for the molecular detection for β-thalassemia and related variants.

    Article Title: Anti-ADAMTS13 antibody and use thereof
    Article Snippet: .. TABLE 2 Primer 1 ggggcggccgcAGAGTGGC SEQ ID NO: 51 CATTACGGCCGGG Primer 2 GGGGAATTCtcatttaccc SEQ ID NO: 52 ggagaccgggagatgg The PCR sample was applied to QIAquick Purification Kit (Qiagen N.V.) for the removal of unreacted primers and enzyme to obtain a purified PCR product. .. The purified PCR product obtained by purification was treated with a restriction enzyme, was separated and purified by agarose gel electrophoresis, and then integrated in a vector. pQEFIP vector (retrovirus vector obtained by using pQCXIP (Takara Bio Inc.) as a backbone in which CMV promoter was replaced with human EF1 alpha promoter) was used for the preparation of a vector containing the heavy chain gene, and pQEFIN vector (retrovirus vector obtained by using pQCXIN (Takara Bio Inc.) as a backbone in which CMV promoter was replaced with human EF1 alpha promoter) was used for the preparation of a vector containing the light chain gene.

    Article Title: Community based countrywide analysis of lactase persistence related genetic variants and their correlation with digestive symptoms in Libya.
    Article Snippet: Genomic DNA was extracted from buccal swabs using Isohelix Buccal DNA Isolation Kit (Cell Projects) according to the manufacturer’s instructions and it was checked by agarose gel electrophoresis. .. Excess salts, primers, and dNTPs were removed by using QIAquick Purification Kit (Qiagen) according to the manufacturer’s protocol before PCR amplification. ..

    Article Title: Unraveling single-molecule reactions via multiplexed in-situ DNA sequencing
    Article Snippet: The top strand was then extended using Q5 DNA Polymerase (Thermo Fisher Scientific) and dNTPs (NEB) using melting, annealing and extension temperatures of 95, 62, and 72 °C, respectively. .. The variant library was purified using the QiAquick purification kit (QIAGEN). ..

    Article Title: Rhizosphere Fungal Community Responses to Nitrogen Addition Reflect Genotypic Versus Trait Variation in the Marsh Sedge Schoenoplectus americanus Over Time
    Article Snippet: .. DNA libraries were purified using the Qiaquick Purification Kit (Qiagen, Germantown, MD, USA) and pooled at 25 ng per sample. .. Sequencing was carried out on the Illumina MiSeq 2 × 250 platform by the Texas A&M AgriLife Genomics and Bioinformatics Service.

    Article Title: DNA Barcoding and Allele-Specific PCR Discrimination of Glasswort Ecotypes from Apulia Region (Southern Italy)
    Article Snippet: .. MatK and rbcL fragments were purified from 100 μL of PCR products using the QIAquick purification Kit (Qiagen, Hilden, Germany) and bidirectionally sequenced at the Eurofins Genomics Sequencing Service (Ebersberg, Germany) with the same PCR primers. ..

    DNA Purification:

    Article Title: Evaluation of a Customized RDB-FTH Panel for β-Globin Variants in a Malaysian Tertiary Center.
    Article Snippet: β-thalassemia is one of the most prevalent autosomal recessive genetic disorders in Malaysia, with an estimated prevalence of 4.5%, encompassing asymptomatic carriers, intermedia and transfusion-dependent β-thalassemia.. Molecular diagnosis has become increasingly important to correlate phenotype to genotype, guiding management, and facilitating genetic counseling, particularly in the prenatal diagnostic setting.. this study evaluated a rapid reversed dot-blotbased flow-through-hybridization (RDB-Fth) assay for the molecular detection for β-thalassemia and related variants.

    Variant Assay:

    Article Title: Unraveling single-molecule reactions via multiplexed in-situ DNA sequencing
    Article Snippet: The top strand was then extended using Q5 DNA Polymerase (Thermo Fisher Scientific) and dNTPs (NEB) using melting, annealing and extension temperatures of 95, 62, and 72 °C, respectively. .. The variant library was purified using the QiAquick purification kit (QIAGEN). ..

    Sequencing:

    Article Title: DNA Barcoding and Allele-Specific PCR Discrimination of Glasswort Ecotypes from Apulia Region (Southern Italy)
    Article Snippet: .. MatK and rbcL fragments were purified from 100 μL of PCR products using the QIAquick purification Kit (Qiagen, Hilden, Germany) and bidirectionally sequenced at the Eurofins Genomics Sequencing Service (Ebersberg, Germany) with the same PCR primers. ..



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    Image Search Results


    Setup of the protocol analysis summarized in various steps.

    Journal: Balkan Journal of Medical Genetics : BJMG

    Article Title: Development and Validation of PCR-Based Ancient DNA Analysis for Italian and Western Balkan Archaeological Collections

    doi: 10.2478/bjmg-2026-0008

    Figure Lengend Snippet: Setup of the protocol analysis summarized in various steps.

    Article Snippet: Demineralized bone material was digested overnight at 56°C using proteinase K (20 mg/mL) and 10% SDS, followed by silica-based purification using QIAquick PCR Purification Kit (Qiagen) [ , , , , , ].

    Techniques: Analysis, Sequencing, DNA Extraction, Sample Prep, Irradiation, Lysis, DNA Purification, Purification, DNA Sequencing, Cloning, Genotyping